FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004693137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74239235
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT38500.021.7164941
TGCGCGA90750.019.8352369
GACGCTA89100.018.177364
ACGCTAC87900.017.99275
GTGCGCG123100.015.3557088
CCCGTAT94000.014.1503771
GTATCGA72600.013.9346844
CGTATCG75100.013.8503393
CCGGTAT121900.013.4837341
ACGTGCG157050.013.154846
TCGCACG73050.012.42079424-25
CGAGTCG76200.012.06064712-13
CGATCGT58100.011.85526574-75
CGCTACT136800.011.6650726
CGGTATT132550.011.5400992
TAACACG168800.011.4539532
GACCGTT72850.010.7593952
TATCGAA158900.010.7302925
CGCGGGT126300.010.5315561
AACACGT196700.010.5036713
Produced by FastQC (version 0.11.5)