FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004693138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74239235
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT42850.020.2849161
TGCGCGA96700.019.4488269
GTGCGCG130600.015.3098718
GACGCTA86200.014.9316084
ACGCTAC81650.014.8330155
CCGGTAT120450.013.7623171
GTATCGA72650.013.72866254
CCCGTAT95800.013.5849491
ACGTGCG152850.013.5788016
CGTATCG82150.013.3551563
TCGCACG76950.012.46994624-25
CGAGTCG80650.012.27817212-13
CGGTATT128000.012.24467852
TAACACG166900.011.7811432
TCAATCG113750.011.3569593
CGCGAGT88800.011.1779910-11
AACACGT193250.011.1087063
CGATCGT59100.010.68952674-75
TATCGAA166550.010.608255
CGTGCGC206200.010.595147
Produced by FastQC (version 0.11.5)