FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56184442
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCAAAACTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT839920.1494933419468685No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC690690.12293260828326817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT62700.047.6252981
CGCAAAA243300.036.054191
ACCGTTG85600.034.8873983
GACCGTT92850.032.0550582
TATCGCG15200.029.5550029
GTCTAAC129800.029.226111
CCGTTGA114950.026.1888354
TAACCCC327750.026.1301569
ACGTGCG149600.025.109148
AACACGT166450.022.9376585
ACTACCG244600.022.1732187
CCGTATG201250.021.5830384
CTAACCC408400.021.2739948
CGTGCGC183950.020.3427059
CTACCGT263900.020.2824088
ACACGTG189150.020.1092746
CGTATGG219350.019.932865
CCGTCAT129750.019.5161291
TACCGTA272200.019.4886449
CACATGC389300.019.2068061
Produced by FastQC (version 0.11.5)