FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77318605
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTTGCAGCAACAAGTTTTTTACCCTCCT1177080.15223761473709982No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAATCATCCATAAC802600.10380425254697755No Hit
GGCGTTTTCTCATGCAACACACATAACTGGTTCAGGACCACGGATAGATAGTCTTCTGCACAGGGCATTCTTTTT792180.10245658208654955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG41800.031.35675848
GTATCGA98550.028.1762774
CCCGTAT110500.027.342891
TCGTATG48200.027.1230345
TATCGAA124600.022.892245
CGTATGC61750.021.33886546
GTTCGAC105400.020.22739629
TCGACCG106100.020.15893631
CGACCGT106600.020.03267932
CGTATCG184850.019.9295273
CCGTATC200000.018.8188362
CTCGTAT70000.018.62691344
GCGATCG63600.018.27740941
TATCGCG11750.018.1994422
ACGTTCG64500.018.1810596
CACGAGT73900.017.87909931
TATCTCG71950.017.45068241
ACGTACG20300.017.33277753
AAGTTCG124400.017.30402227
ACGACTT115050.017.134971
Produced by FastQC (version 0.11.5)