FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77318605
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGG1216380.1573204793335317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT280150.032.88923649
ACGGACA286100.032.65410618
ATTCCGT287200.032.08301553
ACCCGGC297850.031.7323845
CGGACAC303300.030.8582413
TTTCTCG299500.030.84730346
TCTCGAT302350.030.59094648
CGGACAG308100.030.36884719
ATAGCTC314950.029.49829939
CGTGGGT316000.029.02393257
CGCCGTA47950.028.19283546
TCGATTC335150.027.7831850
TTCCGTG336750.027.5056854
CGACCGT42850.027.4958741
GACACGG343200.027.48169315
CACCCGG366600.026.3452344
GGATTGA356750.026.33256125
GTACGCA197250.026.27067650
CGGCCCG366950.026.0361548
GGACACG364000.025.73130414
Produced by FastQC (version 0.11.5)