FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63717679
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACGAGCTCAACAAGTGCAGTTTGTTTCTTGATTTGTCTCTCCTTCTC767430.12044224021405425No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTTGCAGCAACAAGTTTTTTACCCTCCT735230.11538869769565838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA78600.030.0734464
CCCGTAT92350.029.2942331
CGTATCG90850.028.3358523
TATCGAA94950.025.935515
CCGTATC109500.025.42942
ATCGAAG146200.018.7075716
ATCGCGC139100.016.81120767
TAAGCCG157950.015.5473667
CGCGCCA159150.014.75784169
ACGAGCT370200.014.61717529
CATCGCG162850.014.61423566
CGTGTTA162350.014.5094159
GACGAAA162000.014.30913247
TCGCGCC167250.014.22936268
TCACGAG383850.014.16901127
ACGAAAC164600.014.16685848
TATCGTG76950.014.163547556
CGACGAA166200.014.11379346
ACATCGC169950.014.003695565
TACATCG168550.013.91537664
Produced by FastQC (version 0.11.5)