FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66895758
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG55250.038.33171548
TCGTATG65000.032.63631445
CGTATGC72300.029.91359546
GTATCGA77900.026.5683464
CCCGTAT89000.026.250491
CGTATCG86000.026.1517333
TATCTCG82150.024.81500641
CTCGTAT87750.024.09646444
CCGTATC106800.022.7730832
GTATGCC96350.022.23203547
TATCGAA112900.019.55185
ATCTCGT117700.017.78886842
GCCGTCT125750.017.2525351
CGCCCTA173250.016.3649249
ATTTCGC173350.016.23693318
TATCGCG11900.016.23108722
ATCGAAG147250.015.9276986
ATCGCGC126300.015.79091667
CGTCTGA143700.015.26649616
ATCTACG62300.015.11405922
Produced by FastQC (version 0.11.5)