FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67428422
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG52100.034.1004246
TATCGCG39250.030.40753622
TCGTATG58700.030.38469743
CGTATGC68550.026.1190444
GTATCGA92500.023.2706554
CCCGTAT105150.022.6828211
CTCGTAT81100.022.1199142
CGTATCG107750.021.3537563
TATCGAA117800.019.8227565
CCGTATC123750.019.5685062
GTATGCC99050.018.28500445
ATATCGC66050.018.06968921
ACGATCT99950.017.60248837
ATCTACG66450.017.54553222
ATCGCGC117300.017.52636167
ATCACGA109900.016.95043434
ATCGCGG73050.016.71726423
CGTCTGA112700.016.68012816
CTACGTA71650.016.41819824
TACGTAT73500.016.00496325
Produced by FastQC (version 0.11.5)