FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67428422
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA52750.035.24492646
GCCGTAT62050.030.13035447
CGTATCA71900.026.09926849
CGTGTAG72600.025.60659415
CGACCGT39300.022.6240251
TATCGCG39600.022.29706622
GATCGGA85100.022.0888671
TCGTGTA88350.021.47135414
CCGTATC91750.020.79276348
GGTCGCC91850.020.31827243
GCGTCGT101100.019.9249911
TCTCGGT95350.019.78868536
GTCGTGT99000.019.05744713
GATCTCG102500.018.27196934
GTAGATC110100.017.35612531
TGGTCGC111150.017.22492642
ACCGTTG54450.016.5979443
GTCGCCG115750.016.36110344
GTATCAT119000.016.08826450
GTAGGTC90750.016.00312
Produced by FastQC (version 0.11.5)