FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68062291
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTTGCAGCAACAAGTTTTTTACCCTCCT947140.13915781941574668No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACGAGCTCAACAAGTGCAGTTTGTTTCTTGATTTGTCTCTCCTTCTC802370.11788759799460762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG60600.029.71387546
GTATCGA112400.027.5008934
TCGTATG67500.026.72712943
CCCGTAT122150.026.2841431
CGTATCG140850.024.6403143
CGTATGC76400.023.70422444
TATCGAA137400.023.1219795
CCGTATC153700.023.0776042
CTCGTAT84950.021.43942
TATCGCG20750.021.27814722
GTCACGA91800.020.25478429
ATCGAAG193400.018.8870816
TCACGAT101300.018.62588530
GTATGCC102750.017.72604645
CACGATC107750.017.0626131
ATCGCGC182850.016.46936467
ATCTACG55150.016.32438922
CGCGCCA192550.015.71163369
GCCGTCT116050.015.66589749
TCGCGCC196800.015.38952168
Produced by FastQC (version 0.11.5)