FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84554563
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG137800.025.91170722
CGCCGTA101050.023.0117346
GCGTCGT116900.020.18590511
GCCGTAT118300.019.68573647
CGCGATT45600.018.6868131
GATATCG209900.017.66932320
TAACGGT48950.017.40967842
TATACGT47050.017.23145121
TCGCGGC207850.017.19562524
CGTATCA138500.017.1384449
GTATACG50400.017.0448320
ATCGCGG217700.016.62360823
GCATTCG184200.016.2386053
CGGGGGA246000.016.02950510
CATTCGG192950.015.9131934
ATTCGCG55600.015.69844928
TCGCGAT55000.015.6812430
CCGTATA54800.015.61241618
CGTGTAG151900.015.39823315
CGTATAC54850.015.15878119
Produced by FastQC (version 0.11.5)