FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69164431
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA711400.10285633666240962TruSeq Adapter, Index 18 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG162450.040.51804444
TATGCCG169700.038.8304247
CGTCCGC181250.036.44420232
CGTATGC185400.035.5768645
GTCCGCA186750.035.40794833
CTCGTAT185700.035.03625543
GTCACGT197400.033.47924828
GTATGCC200150.033.00788546
GCCGTCT199750.032.2454750
ACACGTC208950.031.8098512
TCCGCAC212400.031.24580234
CGCACAT188200.029.94802936
ACGTCTG222050.029.82446914
AGTCACG222150.029.74949627
CTAACGG33600.029.05605368
TCGGAAG239600.028.8493652
CGTCTGA232300.028.49364915
CACGTCT234400.028.44438413
CCGCACA245200.027.71321335
ACTAACG36000.027.31093467
Produced by FastQC (version 0.11.5)