FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75645709
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG83400.024.32321447
TCGTATG88800.022.57214744
CGTATGC99100.020.226145
TATCGCG32650.019.6529622
TCGACCG58800.017.48401531
CCCGTAT79850.017.222751
TCGCGTA65800.016.98704769
TATCTCG110750.016.88342940
TATCGTG137200.016.87200256
ACGTTCG55300.016.5326526
GTCGTCT275400.016.4613041
CGACCGT63350.016.11934332
GTATCGA70050.016.05734
CGTATCG106100.016.0322553
GTTCGAC64450.016.00480829
TATCGAA84050.015.7207425
CGTGCGA58450.015.582046538
CTCGTAT132800.015.249536543
CGACCGC74850.015.07147955
TCGTCTC284650.015.0128432
Produced by FastQC (version 0.11.5)