FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63253526
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGAACTTAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA1420960.22464518420680613Illumina Single End PCR Primer 1 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA195350.052.41582558
GCCGTAT198450.051.5796159
CGTATCA201950.050.36106561
CCGTATC213900.047.8218360
GTAGATC237750.043.40208443
TAGTGTA241800.042.41843839
GATCTCG256750.040.17703246
ATCTCGG261300.039.50377347
TAGATCT264600.038.8543644
GTATCAT264350.038.74730362
TGGTCGC267400.038.53748354
CGTGTAG270300.038.1625215
GGTCGCC273600.037.9163855
GCGTCGT277700.037.43144211
TCGGTGG283600.036.56745550
TCGCCGT284000.036.41856457
ACTTAGT283850.036.35313436
TCGTGTA286850.036.02084714
CTTAGTG287750.035.74067337
AGTGTAG292300.035.25522640
Produced by FastQC (version 0.11.5)