FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55007417
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1149790.2090245393634826TruSeq Adapter, Index 5 (97% over 41bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG193150.049.42207748
TCGTATG196000.048.7738445
CGTATGC208950.045.9656546
CTCGTAT212700.044.8153344
GTATGCC231850.041.3065547
TATCTCG210100.040.41092741
ATGCCGT276050.034.3677849
TCTCGTA256350.033.45654343
ATCTCGT258900.033.2069742
CGTCTGA316200.030.63674216
GTATCTC278500.030.63459440
CCGTCTT312350.030.16384752
TGGTATC298250.028.73330538
TGCCGTC333500.028.66468850
GTGGTAT303300.028.20897937
GCCGTCT352600.027.10216551
ACGTCTG358900.026.9725215
GGTATCT323150.026.45523339
CACGTCT368850.026.3384514
CGTCTTC360400.026.28586653
Produced by FastQC (version 0.11.5)