FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56293902
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCCAACCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1398520.2484318816627776TruSeq Adapter, Index 6 (97% over 38bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT692320.1229831252415226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG208800.055.15160445
TATGCCG214150.053.66134648
CTCGTAT221300.051.92727344
CGTATGC231450.050.1121246
ACCTATC213300.048.3110738
TATCTCG213350.048.18655841
GTATGCC246100.046.91903347
AACCTAT249850.041.61655837
ATCTCGT248700.041.54543742
CGTCTGA287250.040.72583816
GCCGTCT279400.040.6605351
TCTCGTA255600.040.3699243
CCTATCT266000.038.84342239
CTATCTC270300.038.3148340
CAACCTA308350.037.86070336
CCGTCTT310450.036.5049252
ACGTCTG332000.035.21665615
CACGTCT334600.035.0048714
ATGCCGT340800.033.46646549
GATCGGA367150.032.0928571
Produced by FastQC (version 0.11.5)