FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62545179
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT799360.12780521421163413No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCACACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA673320.10765338124621884TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG112450.050.31469748
TCGTATG120800.046.77966745
CGTATGC126200.045.297446
CTCGTAT131600.042.65223744
GTATGCC154450.036.85585847
GCCGTCT182750.030.73308651
CGTCTGA193000.030.17349416
TCTCGTA179750.028.90464243
CACGTCT232600.025.34809114
ATCTCGT215200.023.98273842
ACGTCTG251000.023.43492715
ACACGTC269050.021.79861313
ATGCCGT267650.021.2551249
CACACGT279250.021.1753512
TGCCGTC288350.019.94452550
GATCGGA297300.019.7260271
CATCTCG278050.019.19448941
CGGAAGA311700.019.0610034
ATCGGAA319150.018.4109172
CCGTCTT314000.018.0736352
Produced by FastQC (version 0.11.5)