FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62545179
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACTTAGCAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA757940.12118280131550986Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA107600.052.70985458
GCCGTAT119400.047.78960859
CGTATCA124900.045.38160361
CCGTATC145850.039.43064560
GTAGATC152150.038.38736343
TCTCGGT152650.037.5157748
CGTGTAG156850.036.90711215
GGTCGCC160350.035.994155
GATCTCG166150.034.73752646
TAGATCT174100.033.46836544
TGGTCGC173350.032.9365854
TCGCCGT174800.032.7423157
ACTTAGC176150.032.57000433
GCGTCGT182350.032.42774211
GTACTTA177700.032.36343431
GTATCAT179700.031.92680562
TCGTGTA183150.031.64514214
ATCTCGG187300.031.01748347
GTGTACT188650.030.6493229
GTGGTCG196450.029.34463953
Produced by FastQC (version 0.11.5)