FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63335734
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT745200.11765869801082592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG94050.044.93648
TCGTATG100500.042.4296645
CTCGTAT113550.037.34065244
CGTATGC126300.033.84426546
GTATGCC146350.029.25473847
CGTCTGA176500.024.96117816
GCCGTCT177600.024.20395951
ATCTCGT165650.023.88832742
TCTCGTA170650.023.1077243
CACGTCT220050.020.30332814
ATGCCGT219050.019.71857349
ACACGTC235050.018.84619313
ACGTCTG235300.018.7528615
CACACGT270400.016.42059912
CATCTCG248300.016.29796241
GATCGGA277550.015.7483271
CCGTCTT282550.015.29914252
TGCCGTC287800.015.24780450
ATCGGAA289200.015.1749722
TCGGAAG308700.014.5737553
Produced by FastQC (version 0.11.5)