FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63335734
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA92100.046.336458
GCCGTAT103000.041.7343259
CGTATCA109350.039.53164361
CCGTATC131400.033.23922360
GTAGATC144850.030.20061143
CGTGTAG148100.029.58443515
TCTCGGT150800.029.07725148
TCGTGTA159150.027.53034814
GGTCGCC161800.026.99379555
GCGTCGT167300.026.70514911
GATCTCG166900.026.50002946
TGGTCGC164100.026.36317854
TCGCCGT170650.025.08847857
GTATCAT175650.024.86559162
GTCGTGT181100.024.30855813
TAGATCT184300.023.7548144
ATCTCGG186100.023.5988747
TCGGTGG198000.022.26767250
GTGGTCG198300.022.04259353
AGCGTCG206000.021.97300310
Produced by FastQC (version 0.11.5)