FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56507083
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGGTCAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA599940.10617076092920953TruSeq Adapter, Index 15 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG104700.045.2424248
TCGTATG112150.042.42159345
CGTATGC115800.041.14405446
CTCGTAT126250.037.3011444
GTATGCC140550.033.84974747
CGTCTGA172550.028.1316116
GCCGTCT179200.026.39465751
ATCTCGT187650.023.82741242
TCTCGTA188500.023.5918543
CACGTCT217500.022.44467714
ATGCCGT215500.022.0606849
GATCGGA232450.021.0062981
GGGTCAA240200.020.4958735
ACGTCTG243850.020.30226915
TGCCGTC248100.019.49567450
GTCAAAT244000.019.13037137
AATCTCG236000.019.00407841
ACACGTC261700.018.66705313
CGGAAGA278200.018.2295864
CACACGT269900.018.04878612
Produced by FastQC (version 0.11.5)