FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56507083
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAGGTCTAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA647660.11461571994434751Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA100350.047.58115458
GCCGTAT108150.044.21330359
CGTATCA112000.042.90909261
CCGTATC135200.036.38807360
GTAGATC139900.034.59887343
GTTAGGT139450.034.4383231
CGTGTAG141950.033.92862315
GTCTAGT142650.033.68963636
TAGTGTA144150.033.55485539
TCTCGGT145150.033.22831348
GGTCGCC148250.032.57988755
TTAGGTC150750.031.94841832
TCGTGTA153700.031.33486214
GATCTCG159200.030.55583846
GCGTCGT158700.030.47805811
CTAGTGT163100.029.9735338
TAGATCT163400.029.85515644
TCTAGTG165950.029.27132637
GTATCAT167350.029.02640262
ATCTCGG171000.028.5280147
Produced by FastQC (version 0.11.5)