FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61089772
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGATTAGGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA1020140.16699031058750718Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA144400.053.51576658
GCCGTAT152650.050.6459359
CGTATCA155300.049.538361
CGATTAG157700.048.67508334
TCGATTA164500.046.74717733
CCGTATC170200.045.85014760
GTAGATC201150.038.98313543
TCTCGGT203100.038.33751748
GATTAGG201150.038.29808435
GGTCGCC202950.038.19555355
TTCGATT212150.036.39387532
CGTGTAG218100.035.77985415
GTATCAT220250.035.25866362
TGGTCGC222000.034.91796554
TTAGGTG224500.034.498737
TCGCCGT224500.034.3600957
TAGATCT229500.034.2426544
GATCTCG228650.034.12901746
ATTAGGT227100.033.9973136
GCGTCGT236250.033.2062311
Produced by FastQC (version 0.11.5)