FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79381082
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC1196850.150772699218184No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC817940.10303966378286454No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG799870.10076330277281934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA447450.035.627144
CTCTAGT456700.034.928216
ACTCTAG475500.033.728625
CTAGTTG503250.031.334088
TCGTATG83100.030.38782345
GTTACCT518650.030.17754714
ACTACCC209250.030.071076
GTACTCT535750.029.7294733
TACTACC213700.029.541745
TGTTACC542250.028.95957813
TGTACTC564900.028.4592782
TTACCTC556700.028.22647715
TATGCCG89350.028.10923448
CTAATGC560050.027.78660821
ACCTCTA578850.027.18809317
TTGTACT597750.026.5095771
TCTAATG595100.026.21380820
TACCTCT627150.025.13270816
CGTATGC101750.025.09051346
TCTAGTT665850.024.8428697
Produced by FastQC (version 0.11.5)