FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79381082
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATC1334270.16808412865926922No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC1299650.16372288803017324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTTG592800.042.824398
GTACTCT624650.041.6318933
TACTCTA630800.041.6307144
TGTACTC635200.041.049032
CTCTAGT636500.040.664766
ACTCTAG662450.039.5145075
TAGTTGT655000.038.9789439
TCTAGTT669800.038.6842657
TTGTACT711900.036.5733761
GTTACCT696950.036.37763614
GTTGTTA701150.036.2165911
CTAATGC723450.034.4441621
TCTAATG729250.034.37836520
CTCTAAT752450.033.5613919
TTGTTAC760800.033.4244112
AGTTGTT783050.032.74579610
TTACCTC786550.032.35648315
GCCGTAT83600.031.19765959
TGTTACC825650.030.69883713
CGCCGTA85900.030.52297858
Produced by FastQC (version 0.11.5)