FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59149656
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA266300.036.571774
CTAGTTG261800.036.201988
GTACTCT283550.034.055323
TGTACTC287900.033.7084162
TAGTTGT286550.033.2557379
CTCTAGT297550.032.5713046
TCTAGTT334000.029.7295047
ACTCTAG333600.029.3696845
GTTGTTA327350.028.92111811
TCTAATG321750.028.90708420
CTAATGC320800.028.85288421
CTCTAAT331750.028.31650419
GTTACCT340300.027.65684514
ACCTCTA350350.026.88211817
TTGTACT363650.026.4447611
TTACCTC358550.026.44156815
TTGTTAC361950.026.20398112
CCTCTAA361650.026.03262718
AGTTGTT385750.024.91830610
GATAGTC248850.023.55467237
Produced by FastQC (version 0.11.5)