FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75660402
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG48400.019.95785948
TCGTATG53900.017.665345
CGTATGC72700.013.57163946
CTCGTAT78750.012.2223444
TACTCTA142850.011.4237844
CCCGTAT73450.011.0474951
GCGGGTT108950.010.9973991
CTCTAGT167550.010.8921096
CGGGCTA55600.010.6817251
TATCTCG87050.010.58183541
CGGGTAT64300.010.578961
CTAGTAC80000.010.43646354
CGGGTGT167250.010.1574971
CGTATCG54450.010.011253
GTCCTAC165200.09.79447741
ACTACCC91800.09.7332486
CGGGGTT239000.09.6653141
TTACCTC169900.09.62466615
CGGGGAT171200.09.62060451
TCGATTG80350.09.57459225
Produced by FastQC (version 0.11.5)