FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68707211
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT42550.021.899251
CGCCGTA41700.019.52483458
GCCGTAT49800.016.55693659
ACCGTTG56700.016.5513693
CGTATCA52850.015.14505561
CCGTTGA64150.014.7905434
CTAGTTG101500.014.0378678
GCGCTAA106150.014.00750442
GTACGTC59300.013.73015331
CGTATGG97300.013.54421112
CCGTATG99500.013.48744611
CGCTAAG110150.013.46820143
CGTGTAG65700.012.70729715
CCGTATC79300.012.26884860
CCGTCAT134450.011.9102871
TACGTCC68550.011.87702732
TAGTTGT124900.011.8498269
TACCGTA130350.011.8307879
TCGATCG90350.011.79868269
GACCGTT78850.011.7722222
Produced by FastQC (version 0.11.5)