FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58661092
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA105550.016.1805134
CTCTAGT128350.013.8696986
TCGACCG34800.013.58169827
GTTCGAC38000.012.347177525
GTTACCT136800.012.28194414
ACTCTAG145800.012.2806955
CGACCGT38050.012.14962628
GCGGGTT87850.011.3915651
TTACCTC155000.011.28466515
CTAATGC152000.011.14449221
TATGCCG35250.011.05942348
CGCGTAT7600.010.8974491
TCTAATG158500.010.88336320
GTCCTAC146350.010.82018341
TGTTACC161600.010.78137713
CTAGTTG163800.010.5733858
ACTACCC71500.010.3257336
TAATGCG38100.010.32269334
CGGGGTT182550.010.3024811
CGCGTTT14750.010.060131
Produced by FastQC (version 0.11.5)