FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58661092
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTTG114800.023.1112448
TACTCTA141300.019.9974584
TAGTTGT149400.018.3578649
TGTACTC159100.017.0467072
CTCTAGT168500.016.4419966
TCTAGTT182350.015.9878437
TCTAATG169000.015.69791320
ACTCTAG183650.015.6680175
GTACTCT172350.015.6541133
CGCGGGT136350.015.4533861
TAGATAG105450.015.24601754
CTCTAAT177600.015.19008319
GATAGTA106850.015.11065356
GTTACCT174150.014.817533514
GTTGTTA181150.014.56894111
GATAGTC118350.014.10836837
CTAATGC187500.014.07545421
GGTTAGA122800.013.59876951
GTTAGAT124400.013.56254252
TTGGACG148400.013.43668530
Produced by FastQC (version 0.11.5)