FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62262315
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG34500.014.79970648
TACTCTA116150.012.0297844
CGGGTAT53850.011.4126921
CGCGTAT8450.011.0321691
CTAGTTG127400.010.9672448
GCGGGTT85750.010.9116141
TCGTATG46700.010.78565445
CGGGCAA90750.010.7289271
CGGGCTA46100.010.6350931
CTCTAGT143850.010.6006716
CGGGTGT155450.010.5056821
CGGGGAT117350.010.4741971
ATTTCGC109400.010.46980518
ACTCTAG145750.010.2494445
CGTATGC52750.010.202641546
CGCCCTA117350.09.99554349
CGTATCG34900.09.78751853
CGGGGAA183500.09.7841051
CGGGGTT206800.09.6834761
CCGACTA26050.09.667747527
Produced by FastQC (version 0.11.5)