FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64433287
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG42000.019.7960448
CTAGTTG152950.017.9094778
CTCTAGT163700.017.57676
TACTCTA169600.016.4364054
CTAGTAC82850.015.44871254
ACTACCC86900.015.4436456
ACTCTAG187100.015.3968785
TTACCTC180400.015.2799115
TCGTATG54650.015.2137945
GTTACCT187100.014.67743514
CGTATGC58850.014.36250546
CTAATGC189950.014.33053621
TGTTACC196650.014.10499613
TCTAGTT242350.012.8402977
CTCGTAT64450.012.6863344
GTCCTAC211700.012.45042141
CGGGTAT60750.012.0482731
TCTAATG231700.011.92699920
CCTAGTA115000.011.36977753
ACCTGAT228150.011.35612133
Produced by FastQC (version 0.11.5)