FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65854768
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG71950.034.4275448
TCGTATG82800.030.66565545
TAATGCG83800.029.9283434
CGTATGC89850.028.72025546
CTCGTAT96600.026.35627444
GTATGCC108700.023.07368347
ATCTCGT111000.019.3312442
CGTCTGA158550.019.25614216
GCCGTCT139000.018.98711651
TGCGCAT123850.017.63579237
ACACGTC175900.017.2587413
CTCTAGT128500.017.235516
ACTAATG153950.016.58228132
AATGCGC158950.015.93392435
CACGTCT192950.015.89458714
TCTCGTA141250.015.50922343
CACACGT199050.015.33816212
ACGTCTG199900.015.2211715
TACTCTA145300.015.150164
CGGGTAT47750.015.0485141
Produced by FastQC (version 0.11.5)