FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65854768
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA75750.037.61900758
GCCGTAT84800.033.88902759
CGTATCA90600.031.68274961
CCGTATC115150.026.33617260
CGTGTAG118550.025.2884115
TCTCGGT115900.025.03486348
GGTCGCC120350.024.50919255
CTAGTTG138100.023.8825158
CGACCGT36600.023.761861
TCGCCGT126400.023.39042357
TGGTCGC125150.023.34863554
GATTAGT119550.023.08575436
TAGTGTA128100.022.59521739
GATCTCG137500.021.80468246
TGTACTC164150.020.8952752
TAGTTGT160700.020.7814259
GTCGCCG142700.020.71863656
GTAGATC145200.020.5530843
CTCTAGT168500.020.3940566
GCGTCGT162400.020.2658911
Produced by FastQC (version 0.11.5)