FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66142586
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT42200.026.248341
ACCGTTG55800.019.8467963
GACCGTT69850.016.1033342
ACGGACA142100.015.05268418
GTCTAAC102350.014.8345391
GTACGCA145000.014.06161450
CCGTCAT110250.013.990661
CTAGTTG99700.013.66830358
TACGCAC150200.013.55182351
CGGACAC159900.013.54950913
CGGACAG163700.013.46692819
CGTATGG80050.013.27400912
CCGTATG80900.013.17718511
CCGTTGA86950.013.1730034
AGTACGC155500.013.15643849
CGAGTCG112000.013.09127515
CCGGTAC156850.012.95514160
GCGCTAA111200.012.81317142
CTCGATT161200.012.79823849
ACGCACG161700.012.69473452
Produced by FastQC (version 0.11.5)