FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65393783
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG35450.017.12600748
GCGGGTT96950.013.5867271
CGTATCG43700.013.5035243
CGGGTAT47950.013.1193841
CCCGTAT67250.012.8168961
GTATCGA46600.012.3669614
CGGGCTA45000.012.3575091
CGGGGAT115850.012.1801731
TCGTATG50100.012.11810345
CGGGGTT195900.011.9222531
TACTCTA110850.011.5185654
TACCGCG71250.011.32897713
GCCGTTA71600.011.2254220
CCGTTAA74150.010.839869521
CTAGTTG116800.010.7502428
CCGTATC66550.010.6827512
CGTATGC60400.010.67983146
CGGGTGT171800.010.6006281
CGGGTTA38550.010.4582031
CGGGCAT138500.010.3890081