FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58370668
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTTG151850.029.3989988
CGCCGTA39500.024.80369658
TGTACTC190500.023.9531352
ACTCTAG196000.023.890675
TAGTTGT199000.022.9533129
TACTCTA214150.022.3974554
GCCGTAT45050.021.90187659
CTCTAGT212950.021.6973926
TCTAGTT222150.021.4156387
GTTACCT212650.020.84648914
GTTGTTA220350.020.43145811
GTACTCT224850.020.3954163
CTCTAAT218850.020.20868919
TCTAATG215250.020.13009320
GATAGTC149750.018.54657437
CGTATCA52600.018.49581561
GATAGTA147750.018.18884756
TAGATAG151600.017.9317354
CGCGGGT126050.017.9168431
CTAATGC249950.017.76334221
Produced by FastQC (version 0.11.5)