FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59061461
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTTG169250.030.269788
TGTACTC200650.026.1828022
CTCTAGT204450.025.8532736
TAGTTGT209300.024.7742949
TACTCTA226900.024.2532564
CGCCGTA44150.023.75422158
TCTAGTT242250.022.5578337
GTTACCT228600.022.38069214
TCTAATG228850.022.03953620
ACTCTAG250800.021.3504985
GTTGTTA242750.021.11888911
GTACTCT248350.021.070543
CTCTAAT249000.020.61620919
GCCGTAT52500.020.30477559
CGTATCA53500.019.53876161
CTAATGC269800.018.6432521
GATAGTA166850.018.60928256
GATAGTC173200.018.38486937
TAGATAG169050.018.32628854
TTGTTAC283550.018.1774312
Produced by FastQC (version 0.11.5)