FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54174335
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTTG101750.020.2079668
CGCCGTA40400.019.12792258
GCCGTAT47650.016.65200459
TAGTTGT139550.015.0061649
CGTATCA53100.014.35835161
GCGCTAA98450.014.26196942
TGTACTC150500.014.1275712
TACTCTA173150.014.0279124
CTCTAAT152450.013.6909119
CTCTAGT157650.013.6344286
GTACTCT159200.013.3988863
TCTAATG150300.013.31293420
CGCTAAG105300.013.30143343
TCTAGTT183200.013.257237
CCGTCAT115350.013.1960611
GTTACCT158900.012.89647314
TAGATAG105800.012.84771254
CGCGGGT89800.012.8378831
GATAGTA104350.012.82786856
GATAGTC106000.012.82322237
Produced by FastQC (version 0.11.5)