FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693207

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693207
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61964260
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG34150.023.73441148
TACTCTA118850.018.9508174
TCGTATG43000.018.93095845
CGTATGC46650.017.89339346
CTCTAGT136750.016.6210866
CGCGTAT6650.016.6148471
CCGACTA44050.016.52108627
CGACTAA49200.014.86184728
CGGGCTA46350.014.7497081
CTAGTTG152900.014.1218058
CTCGTAT57450.013.98927744
GCGGGTT99950.013.9216361
ACAACCG55250.013.54655223
CGGGTAT49300.013.4469051
TCTAATG164300.013.28938820
CTAATGC164250.013.20943121
CGGGGAT112150.013.20766451
TCGATTG109150.013.05051525
CTAGTAC71700.013.03621454
CGTATCG68300.012.928173
Produced by FastQC (version 0.11.5)