FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65868606
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG53250.023.1881848
TACTCTA237950.023.0381664
TCGTATG55200.022.49445
ACTCTAG259800.021.3874995
CTCTAGT270350.020.6932036
ACTACCC131700.020.6368646
CTAGTTG261200.020.5201768
CTAATGC258300.020.1764321
CTAGTAC138400.019.35086454
GTTACCT276950.019.2660314
TGTTACC280300.019.03576913
TTACCTC282100.019.02434715
TACTACC147150.018.4466725
CGTATGC68650.018.08694546
GTACTCT318150.017.220553
TCTAATG308350.017.20349320
ACCTGAT325500.016.54076233
CCTATGC167200.016.23460445
TCTAGTT364550.016.0651157
CTCGTAT78000.016.05148544
Produced by FastQC (version 0.11.5)