FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65868606
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTTG271150.033.488358
TACTCTA316500.030.292514
GTACTCT313600.029.7035333
CTCTAGT316500.029.5078076
TAGTTGT323750.028.2821149
TGTACTC338200.027.6367172
CGCCGTA47450.026.68367458
TCTAGTT356450.026.5297497
ACTCTAG357450.026.465165
GTTACCT347100.026.06138814
CTCTAAT357650.025.28282419
CTAATGC354800.025.13583821
TCTAATG359350.024.96158620
GTTGTTA364250.024.93863511
TTGTTAC394500.023.07872412
TGTTACC405500.022.44415913
TTGTACT415550.022.3116171
GCCGTAT58250.022.03245259
CCTCTAA413750.021.98814618
AGTTGTT435100.021.2900310
Produced by FastQC (version 0.11.5)