FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62079221
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTTG143250.024.8060288
CGACCGT44300.021.7323041
CTCTAGT168150.021.5844446
TACTCTA187450.020.7976684
TAGTTGT179050.020.0198489
TGTACTC185950.019.8350322
CTCTAAT191500.018.66411419
CGCGGGT116500.018.6307331
TCTAATG193100.017.97344820
CGCCGTA36450.017.69942358
ACTCTAG223500.017.2887085
ACCGTTG55950.017.142193
TCTAGTT240250.016.9162437
GTTACCT213450.016.72881314
GTACTCT223100.016.6702023
GATAGTC133700.016.4370337
GTTGTTA222150.016.05815111
GATAGTA134150.015.94466256
GCCGTAT43700.015.39459159
GGTTAGA141150.015.12951951
Produced by FastQC (version 0.11.5)