FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693222

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693222
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58446304
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTTG96900.017.9104928
CGACCGT37900.017.6616251
CGCGGGT107550.016.907071
GCGCTAA89400.015.24345842
CGCTAAG95300.014.26353743
TGTACTC134650.013.9647332
CCGTATG80300.013.74984211
ACCGTTG49100.013.209553
GCGGGTC143900.013.0910462
CTCTAGT146600.013.0136826
TACTCTA159650.012.9654644
CGGGTCT142550.012.82685853
TAGTTGT141900.012.7655699
TACCGTA113650.012.5991719
CCGTCAT114650.012.5797011
TTGGACG135050.012.46644130
CCGTTGA53600.012.22914
TCTAATG145300.011.9427220
GTTACCT148300.011.72605514
CGTATGG94400.011.58643512
Produced by FastQC (version 0.11.5)