FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55785094
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG46500.014.0218813
TATGCCG33800.012.96233648
TCGTATG34500.012.69933145
GTATCGA51550.012.1797994
CGGGTAT45100.011.9700821
CCCGTAT72550.011.4001241
CGGGGTT175250.011.2752761
GCGGGTT74100.011.0682561
CGGGGAT104500.010.8950371
CCGTATC70250.010.7055242
TATCGAA61250.010.7013725
CGGGTGT120500.010.4822591
CGCGTTT16100.010.3172611
CGGGCAA82550.09.9352861
ATCGCGC59000.09.82394367
CGCGTAA11050.09.708421
CGGGTTT97200.09.6839321
CGGGCTA44050.09.6629181
CGGGATA38900.09.6077841
CGGGTTA31350.09.4931421
Produced by FastQC (version 0.11.5)