FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55785094
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT35950.015.0705081
CTAGTTG95200.014.6414328
CGCCGTA30400.014.071959558
TACTCTA123650.013.0853444
CGCGGGT94350.011.9600511
GCCGTAT37000.011.93478759
CTCTAGT137000.011.2312716
TAGTTGT135100.010.8280359
TTGGACG99750.010.65232230
TCTAGTT180650.010.31265457
TACCGTA56950.010.2989839
ACCGTTG52350.010.1487023
CGACAAT74300.09.9392161
CGTATCA44350.09.87908161
TCGATCG51000.09.876183569
TGTACTC152650.09.6981212
GTCGTAA24300.09.6540395
CCGTTGA55550.09.6261834
GTACTCT159800.09.5854533
GATAGTA91600.09.41566556
Produced by FastQC (version 0.11.5)