FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66289311
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTTG161350.023.5199958
TACTCTA192350.020.5364864
CTCTAGT193750.019.942946
TAGTTGT205300.018.8546039
GTACTCT211000.017.9855233
ACTCTAG222650.017.9741425
TGTACTC220950.017.5682472
CGACCGT40050.017.1443251
TCTAATG220500.016.66302520
CTAATGC227100.016.33067921
CTCTAAT233450.016.0490319
GTTACCT231000.016.01018314
TCTAGTT280250.015.3878257
GTTGTTA251150.015.12403511
GATAGTC159400.014.50104737
GATAGTA159400.013.95996356
CGCCGTA40750.013.79980258
TAGATAG165350.013.58283154
TTGTTAC283400.013.39079412
GGTTAGA173050.013.17781451
Produced by FastQC (version 0.11.5)