FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74083147
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG66250.018.22484227
TCGTATG47850.017.73585345
GTTCGAC71550.016.87485325
CGACCGT71350.016.87380628
TATGCCG50350.016.85522348
CGCGTAT9050.014.5632261
AACGGAT55700.014.55439837
CGTATGC59300.014.31130846
CTCGTAT61250.013.74303544
AAGTTCG91800.013.64098123
GCGGGTT91000.013.1873421
TTCGACC96000.012.82858126
CGCCCTT103500.012.3654111
TACTCTA158450.012.3448034
CTCTAGT174500.011.7823646
GTCGCTA81750.011.60450327
ATTAGCG117300.011.1166888
CGCGTTT20050.010.8980481
CTAATGC167300.010.82542621
CGCTACT31150.010.5776241
Produced by FastQC (version 0.11.5)