FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74083147
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTTG149700.021.8934978
TACTCTA183500.018.7632144
CGCCGTA48600.018.10158758
GCCGTAT51150.017.46895259
TAGTTGT193100.017.3123049
CGTATCA55400.016.0042861
GTACTCT208250.015.854033
TCTAATG208350.015.25031820
TCTAGTT249000.014.949787
CTAATGC212600.014.94545521
CTCTAAT227650.014.32111719
TGTACTC232650.014.2970822
GTTGTTA235250.013.96113111
GTTACCT233250.013.79981814
GTACGCA110950.013.37070350
GTCTAAC114300.013.3428761
CCGTATC75700.013.17087860
GCGCGAA85250.012.94995858
GATAGTA152400.012.2242356
TTGTTAC269600.012.15673712
Produced by FastQC (version 0.11.5)