FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67687988
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA38600.020.64623336
TATGCCG43600.018.3587748
TACTCTA139800.015.9666164
TCGTATG54700.014.63228945
CTCTAGT161750.014.5676016
CGTATGC59000.013.85823646
CGCGTTT17400.013.4873691
ACTCTAG174350.013.2773745
CGCGTAT8650.012.7673681
CCGCGAA67050.012.34890735
TTACCTC182150.012.08378815
GTTACCT186400.011.7342414
CTAGTTG189850.011.5210628
CGGGTAT53400.011.5039311
CTAATGC184900.011.43776821
TGTTACC192950.011.38960113
CTCGTAT70450.011.26311344
TCTAATG190550.011.22528920
GTCCTAC195950.011.05679341
TCTAGTT239150.010.8338647
Produced by FastQC (version 0.11.5)